6. Organization of the protruding Lamellipodium
An example of correlative light microscopy and conventional electron microscopy is shown in the Movie, Figure 6-1 and the electron micrograph, Fig. 6-2. (From Koestler et al., 2008).
Figure 6-1: Video frames of a cell expressing GFP-actin (green) and RFP-VASP (red; see section ) that was arrested with a mixture of glutaraldehyde and Triton X-100 at the time indicated (FIX). From Koestler et al. Nature Cell Biol. 2008.
(Movie 1.43Mb) Click on picture to watch movie
Figure 6-2: Electron micrograph of the protruding zone of the cell shown in Figure 6-1, showing the diagonal network of actin filaments in lamellipodia and the bundles of actin filaments that make up the microspikes and filopodia. From Koestler et al., 2008.
Click on picture to enlarge
Figure 6-3: Enlargement of region off from top right in Fig. 6-2 showing details of the lamellipodium actin meshwork and the bundled filaments in filopodia.
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